Association between ADIPOQ (rs1501299) SNP with Insulin Resistance in Indonesian Type 2 Diabetes Mellitus Patients

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• Penulis: Donny Nauphar, Robby Irham Maulana Alfaqih, Gara Samara Brajadenta, Tiar M Pratamawati
• Identitas: International Journal of Technology (IJTech) | Vol 14, No 7 (2023) Pages : 1578-1585 | p-ISSN : 2086-9614 e-ISSN : 2087-2100 | Publisher: Faculty of Engineering, Universitas Indonesia | DOI : https://doi.org/10.14716/ijtech.v14i7.6691 | Scopus Q2
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  Abstract Insulin resistance is an important aspect of metabolic endocrine disorder, and adiponectin functions as an insulin-sensitizer. Changes in adiponectin levels are associated with alterations in insulin sensitivity. Insulin resistance results from various variables that contributes to abnormalities in insulin signaling, including a decrease in adiponectin levels. Genetics is recognized as one of the key elements influencing adiponectin levels, with investigations showing that ADIPOQ SNP can impact insulin sensitivity and plasma adiponectin levels. Therefore, this study aimed to examine association between ADIPOQ gene polymorphism in patients with type 2 diabetes mellitus (DM) and insulin resistance level. A case-control study was conducted with 60 participants recruited from Sunyaragi Community Health Center in Cirebon, West Java. Data were collected using fasting blood glucose (mg/dl) and Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP). The results showed that the genotype frequency of SNP in the case group was GG = 12 (40%), GT = 16 (53.33%), TT = 2 (6.67%). Meanwhile, in the control group, it was observed to be GG = 18 (60%), GT = 11 (36.67%), and TT = 1 (3.33%). Statistically analysis showed a significant association between +276 G/T polymorphism and type 2 DM. This concluded that individuals with polymorphism are at higher risk of developing type 2 DM. Keywords Adiponectin; ADIPOQ; Insulin Resistance; SNP

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